Coding

Part:BBa_K4258011:Design

Designed by: Jake Schumacher   Group: iGEM22_NDSU   (2022-09-26)


His-meffRed-Linker-CBDfimi


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 565
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 309
    Illegal NgoMIV site found at 774
    Illegal NgoMIV site found at 786
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 16
    Illegal BsaI.rc site found at 1109


Design Notes

Bpi1 and Bsa1 sites added to the ends for Golden Gate Cloning.


Source

MeffRed from BBa_K1033920 Cellulose Binding Domain from BBa_K863101

References